software version r 2014a Search Results


incucyte zoom software  (Sartorius AG)
99
Sartorius AG incucyte zoom software
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Incucyte Zoom Software, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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incucyte zoom software - by Bioz Stars, 2026-03
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commercial software matlab version 2014a  (MathWorks Inc)
90
MathWorks Inc commercial software matlab version 2014a
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Commercial Software Matlab Version 2014a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
commercial software matlab version 2014a - by Bioz Stars, 2026-03
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custom-developed software package  (MathWorks Inc)
90
MathWorks Inc custom-developed software package
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Custom Developed Software Package, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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winfolia software  (Regent Instruments)
90
Regent Instruments winfolia software
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Winfolia Software, supplied by Regent Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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16-slice helical ct somatom sensation 64  (Siemens Healthineers)
90
Siemens Healthineers 16-slice helical ct somatom sensation 64
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
16 Slice Helical Ct Somatom Sensation 64, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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software package rdrobust  (STATA Corporation)
90
STATA Corporation software package rdrobust
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Software Package Rdrobust, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
software package rdrobust - by Bioz Stars, 2026-03
90/100 stars
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matlab software  (MathWorks Inc)
90
MathWorks Inc matlab software
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Matlab Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matlab software/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
matlab software - by Bioz Stars, 2026-03
90/100 stars
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xlstat 2014 software  (Addinsoft inc)
90
Addinsoft inc xlstat 2014 software
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Xlstat 2014 Software, supplied by Addinsoft inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xlstat 2014 software/product/Addinsoft inc
Average 90 stars, based on 1 article reviews
xlstat 2014 software - by Bioz Stars, 2026-03
90/100 stars
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matlab 2014a  (MathWorks Inc)
90
MathWorks Inc matlab 2014a
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Matlab 2014a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab 2014a - by Bioz Stars, 2026-03
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software vbspt version 1.1  (MathWorks Inc)
90
MathWorks Inc software vbspt version 1.1
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Software Vbspt Version 1.1, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
software vbspt version 1.1 - by Bioz Stars, 2026-03
90/100 stars
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vbspt, version 1.1  (MathWorks Inc)
90
MathWorks Inc vbspt, version 1.1
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Vbspt, Version 1.1, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vbspt, version 1.1 - by Bioz Stars, 2026-03
90/100 stars
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matlab r2014a  (MathWorks Inc)
96
MathWorks Inc matlab r2014a
Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in <t>Incucyte</t> ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Matlab R2014a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in Incucyte ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).

Journal: Cancers

Article Title: DNA Replication Licensing Protein MCM10 Promotes Tumor Progression and Is a Novel Prognostic Biomarker and Potential Therapeutic Target in Breast Cancer

doi: 10.3390/cancers10090282

Figure Lengend Snippet: Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in Incucyte ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).

Article Snippet: Data were analyzed using the IncuCyte Zoom software (version 2014a Essen Bioscience, Ann Arbor, MI, USA), which quantified cell surface area coverage as confluence values.

Techniques: Expressing, Western Blot, Flow Cytometry, Software, MTT Assay, Migration, Cell Migration Assay, Colony Assay